Western blot analysis of anti-Integrin Alpha V/ITGAV antibody (M01561-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Integrin Alpha V/ITGAV antigen affinity purified monoclonal antibody (M01561-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Alpha V/ITGAV at approximately 130 kDa. The expected band size for Integrin Alpha V/ITGAV is at 116 kDa.
IF analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:100. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IHC analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-Integrin Alpha V/ITGAV antibody (M01561-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Integrin Alpha V/ITGAV antigen affinity purified monoclonal antibody (M01561-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Alpha V/ITGAV at approximately 130 kDa. The expected band size for Integrin Alpha V/ITGAV is at 116 kDa.
IF analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:100. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IHC analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of anti-Integrin Alpha V/ITGAV antibody (M01561-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human SiHa whole cell lysates,
Lane 7: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Integrin Alpha V/ITGAV antigen affinity purified monoclonal antibody (M01561-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Alpha V/ITGAV at approximately 130 kDa. The expected band size for Integrin Alpha V/ITGAV is at 116 kDa.
IF analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in an immunocytochemical section of A549 cells. The section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:100. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IHC analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Integrin Alpha V/ITGAV using anti-Integrin Alpha V/ITGAV antibody (M01561-2).
Integrin Alpha V/ITGAV was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Integrin Alpha V/ITGAV Antibody (M01561-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
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