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Anti-MCM7 Antibody (Clone#3H11)

Mouse monoclonal antibody

说明书

筛选器: All WB IHC ICC/IF FCM

M01649-3

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Figure 1. Western blot analysis of MCM7 using anti-MCM7 antibody (M01649-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Hela whole cell lysates,
    Lane 2: MCF-7 whole cell lysates,
    Lane 3: MDA-MB-231 whole cell lysates,
    Lane 4: C6 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-MCM7 antigen affinity purified monoclonal antibody (M01649-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.

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  • Figure 2. Western blot analysis of MCM7 using anti-MCM7 antibody (M01649-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human K562 whole cell lysates,
    Lane 3: human Jurkat whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates,
    Lane 5: human 293T whole cell lysates,
    Lane 6: human U2OS whole cell lysates,
    Lane 7: human A549 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-MCM7 antigen affinity purified monoclonal antibody (M01649-3) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-mouse IgG (H+L) secondary antibody (Catalog # BA1151). A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.

    all(7)
  • Figure 3. IHC analysis of MCM7 using anti-MCM7 antibody (M01649-3).
    MCM7 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-MCM7 Antibody (M01649-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

    all(7)
  • Figure 4. IHC analysis of MCM7 using anti-MCM7 antibody (M01649-3).
    MCM7 was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-MCM7 Antibody (M01649-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

    all(7)
  • Figure 5. IHC analysis of MCM7 using anti-MCM7 antibody (M01649-3).
    MCM7 was detected in a paraffin-embedded section of Adenocarcinoma of the colon tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-MCM7 Antibody (M01649-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1022) as the chromogen.

    all(7)
  • Figure 6. IF analysis of MCM7 using anti-MCM7 antibody (M01649-3).
    MCM7 was detected in an immunocytochemical section of PC-3 cells. The section was incubated with mouse anti-MCM7 Antibody (M01649-3) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(7)
  • Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-MCM7 antibody (M01649-3).
    Overlay histogram showing MCF-7 cells stained with M01649-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM7 Antibody (M01649-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

    all(7)

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产品名称
Anti-MCM7 Antibody (Clone#3H11)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CDABP0042; CDC47; CDC47 homolog; MCM2; MCM7; P1.1 MCM3; P1CDC47; P85MCM; PNAS 146
产品类型
Monoclonal
检验物种
human, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
MCM7
克隆号
3H11
宿主
Mouse
抗体亚型
IgG1
免疫原
E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7.
计算分子量
81 kDa
实际分子量
81 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
protein G/A purified
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
Uniprot ID
P33993  
文献引用格式
MCM7 Antibody (Clone#3H11) (Boster Biological Technology, Wuhan, China. Catalog#M01649-3)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
minichromosome maintenance complex component 7
蛋白名全称
DNA replication licensing factor MCM7
推荐稀释比
Western blot (WB):1:500-2000
Immunocytochemistry/Immunofluorescence(ICC/IF):1:50-400
Immunohistochemistry (IHC):1:50-400
Flow Cytometry (Fixed):1:50-200
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-MCM7 Antibody (Clone#3H11)

    筛选器: All WB IHC ICC/IF FCM

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