Anti-MFN1 Antibody (Clone#3H3)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF

M02172-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MFN1 using anti-MFN1 antibody (M02172-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-MFN1 antigen affinity purified monoclonal antibody (M02172-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MFN1 at approximately 84 kDa. The expected band size for MFN1 is at 84 kDa.
all(2)
ICC/IF analysis of MFN1 using anti-MFN1 antibody (M02172-1).
MFN1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-MFN1 Antibody (M02172-1) at a dilution of 1:100. Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(2)

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产品名称

Anti-MFN1 Antibody (Clone#3H3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Fzo homolog; hfzo1; hfzo2; MFN1; mitofusin 1; Transmembrane GTPase MFN1

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF

基因名称

MFN1

克隆号

3H3

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.

计算分子量

84 kDa

实际分子量

84 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80 90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed,and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion,and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers,either with itself or MFN 2,and to undergo ubiquitination by MARCH5. MFN 1 has two key domains. One is a coiled°Coil region that mediates MFN 1: MFN 1/2 binding,and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Uniprot ID

Q8IWA4

RRID

AB_3081862

文献引用格式

MFN1 Antibody (Clone#3H3) (Boster Biological Technology, Wuhan, China. Catalog#M02172-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

mitofusin 1

蛋白名全称

Mitofusin-1

实验方案>

实验步骤

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