Anti-hnRNP-E1/PCBP1 Antibody (Clone#OTI3B9)

Mouse monoclonal antibody

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筛选器： All IHC WB

M02636-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M02636-2)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCBP1.
all(2)

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产品简介>

产品名称

Anti-hnRNP-E1/PCBP1 Antibody (Clone#OTI3B9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha CP1; hnRNP E1; hnRNP X; hnRNP-E1; HNRPE1; HNRPX; PCBP1; poly(rC) binding protein 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

PCBP1

克隆号

OTI3B9

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 1-225 of human PCBP1(NP_006187) produced in E.coli.

计算分子量

37 kDa

实际分子量

37.3 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]

Uniprot ID

Q15365

文献引用格式

hnRNP-E1/PCBP1 Antibody (Clone#OTI3B9) (Boster Biological Technology, Wuhan, China. Catalog#M02636-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Poly(rC-binding protein 1|Alpha-CP1|Heterogeneous nuclear ribonucleoprotein E1|hnRNP E1|Nucleic acid-binding protein SUB2.3

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