Anti-HSD3B1 Antibody (Clone#3F3C/7F9C8)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF

M02856

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (M02856). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-HSD3B1 antigen affinity purified monoclonal antibody (M02856) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSD3B1 at approximately 42 kDa. The expected band size for HSD3B1 is at 42 kDa.
all(2)
IF analysis of HSD3B1 using anti-HSD3B1 antibody (M02856).
HSD3B1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-HSD3B1 Antibody (M02856) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(2)

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产品名称

Anti-HSD3B1 Antibody (Clone#3F3C/7F9C8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

hydroxy-delta-5-steroid dehydrogenase; 3 beta- and steroid delta-isomerase 1; 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1; HSD3B1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

HSD3B1

克隆号

3F3C/7F9C8

宿主

Mouse

抗体亚型

IgG2b

免疫原

E.coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

Uniprot ID

P14060

文献引用格式

HSD3B1 Antibody (Clone#3F3C/7F9C8) (Boster Biological Technology, Wuhan, China. Catalog#M02856)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

蛋白名全称

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

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实验步骤

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