Anti-Alpha Actinin/ACTN2 Antibody (Clone#21A00)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

M03673-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Alpha Actinin/ACTN2 antibody (M03673-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Actinin/ACTN2 antigen affinity purified monoclonal antibody (M03673-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha Actinin/ACTN2 at approximately 104 kDa. The expected band size for Alpha Actinin/ACTN2 is at 104 kDa.
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产品名称

Anti-Alpha Actinin/ACTN2 Antibody (Clone#21A00)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

actinin, alpha 2; ACTN2; Alpha actinin 2; CMD1AA; F actin cross linking protein

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

ACTN2

克隆号

21A00

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Alpha actinin 2

计算分子量

104 kDa

实际分子量

104 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Alpha-actinin 2 is a protein which in humans is encoded by the ACTN2 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P35609

文献引用格式

Alpha Actinin/ACTN2 Antibody (Clone#21A00) (Boster Biological Technology, Wuhan, China. Catalog#M03673-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

actinin alpha 2

蛋白名全称

Alpha-actinin-2

实验方案>

实验步骤

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