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Anti-RNH1 Antibody (Clone#OTI4G4)

Mouse monoclonal antibody

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M04147-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RNH1 antibody, and then analyzed by flow cytometry.

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  • Flow cytometric Analysis of Hela cells, using anti-RNH1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  • Anti-RNH1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNH1 .

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  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RNH1 mouse monoclonal antibody. (M04147-2)

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  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNH1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNH1.

    all(5)

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产品名称
Anti-RNH1 Antibody (Clone#OTI4G4)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Placental RNase inhibitor; PRI; RAI; Ribonuclease inhibitor; RNH; RNH1
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, FCM
基因名称
RNH1
克隆号
OTI4G4
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.
计算分子量
50 kDa
实际分子量
50 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo. [supplied by OMIM]
Uniprot ID
P13489  
文献引用格式
RNH1 Antibody (Clone#OTI4G4) (Boster Biological Technology, Wuhan, China. Catalog#M04147-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
ribonuclease/angiogenin inhibitor 1
蛋白名全称
Ribonuclease inhibitor
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow cytometry (FCM):1:100

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