Anti-AMPK Beta 2/PRKAB2 Antibody (Clone#6G1)

筛选器： All FCM WB

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  Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody (M05077).Overlay histogram showing PC-3 cells stained with M05077 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (M05077, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Western blot analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (M05077). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human 293T whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human A375 whole cell lysates,
  Lane 6: human A431 whole cell lysates,
  Lane 7: human U2OS whole cell lysates,
  Lane 8: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AMPK Beta 2/PRKAB2 antigen affinity purified monoclonal antibody (M05077) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Beta 2/PRKAB2 at approximately 34 kDa. The expected band size for AMPK Beta 2/PRKAB2 is at 30 kDa.

• 

  Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody (M05077).Overlay histogram showing PC-3 cells stained with M05077 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (M05077, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of AMPK Beta 2/PRKAB2 using anti-AMPK Beta 2/PRKAB2 antibody (M05077). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human 293T whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human A375 whole cell lysates,
  Lane 6: human A431 whole cell lysates,
  Lane 7: human U2OS whole cell lysates,
  Lane 8: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-AMPK Beta 2/PRKAB2 antigen affinity purified monoclonal antibody (M05077) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Beta 2/PRKAB2 at approximately 34 kDa. The expected band size for AMPK Beta 2/PRKAB2 is at 30 kDa.