Anti-COX4I1 Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of anti- COX4I1 antibody (M05442-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: human HEK293 whole cell lysates,
  Lane 4: human T47D whole cell lysates,
  Lane 5: human Caco-2 whole cell lysates,
  Lane 6: human K562 whole cell lysates,
  Lane 7: human Hela whole cell lysates,
  Lane 8: rat brain tissue lysates,
  Lane 9: mouse brain tissue lysates.
  Use mouse anti- COX4I1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for COX4I1 at approximately 17KD. The expected band size for COX4I1 is at 20KD.

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  Figure 2. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human colon cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

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  Figure 3. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

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  Figure 4. IHC analysis using anti- COX4I1 antibody (M05442-1). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.

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  Figure 5. Flow Cytometry analysis of U937 cells using anti-COX4I1 antibody (M05442-1).
  Overlay histogram showing U937 cells stained with M05442-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COX4I1 Antibody (M05442-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.