Figure 1. Western Blotting result
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells.
Figure 2. Specificity Test result
RM157 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.
Figure 3. Dot Blot result
A Peptide dotblot shows RM157 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity with nonmodified Lysine 79 (H3K79Ctrl), monomethylated Lysine 79 (K79me1) or dimethylated Lysine 79 (K79me2).