Anti-ABAT Antibody (Clone#OTI3D2)

Mouse monoclonal antibody

说明书

筛选器： All WB IHC

M07133-3

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期： 5-7天

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Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.
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Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M07133-3)
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Figure 3. Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M07133-3)
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产品简介>

产品名称

Anti-ABAT Antibody (Clone#OTI3D2)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ABAT; GABA aminotransferase; GABA AT; GABA T; GABA transaminase; GABAT; L AIBAT

产品类型

Monoclonal

检验物种

human,mouse,rat

应用范围

WB, IHC

基因名称

ABAT

克隆号

OTI3D2

抗体来源

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.

计算分子量

56 kDa

实际分子量

53.2 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot ID

P80404

文献引用格式

ABAT Antibody (Clone#OTI3D2) (Boster Biological Technology, Wuhan, China. Catalog#M07133-3)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

4-aminobutyrate aminotransferase

蛋白名全称

4-aminobutyrate aminotransferase, mitochondrial

实验方案>

实验步骤

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