Anti-Flag Tag Antibody (Clone#1E6)

Mouse monoclonal antibody

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筛选器： All WB

M30971

50μl ￥1280 100μl ￥2180
货期：现货

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Western blot analysis of different concentration of Anti-Flag Tag(1E6) Monoclonal Antibody (M30971)
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins
all(1)

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产品名称

Anti-Flag Tag Antibody (Clone#1E6)

规格/价格

50μl/1280 100μl/2180

产品类型

Monoclonal

检验物种

All species

应用范围

克隆号

1E6

宿主

Mouse

抗体亚型

IgG2b

免疫原

This antibody is produced by immunizing animals with a synthetic Flag tag (DYKDDDDK) peptide corresponding to KLH.

实际分子量

45 kDa

成分

500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

浓度

0.5mg/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.

文献引用格式

Flag Tag Antibody (Clone#1E6) (Boster Biological Technology, Wuhan, China. Catalog#M30971)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

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Anti-Flag Tag Antibody (Clone#1E6)

筛选器： All WB

Western blot analysis of different concentration of Anti-Flag Tag(1E6) Monoclonal Antibody (M30971)
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins