Western blot (WB): | 1:1000-5000 |
Immunohistochemistry (IHC): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:80 |
Western blot analysis of anti-MGEA5/OGA antibody (M32463). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MGEA5/OGA antigen affinity purified monoclonal antibody (M32463) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MGEA5/OGA at approximately 130 kDa. The expected band size for MGEA5/OGA is at 103 kDa.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Western blot analysis of anti-MGEA5/OGA antibody (M32463). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MGEA5/OGA antigen affinity purified monoclonal antibody (M32463) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MGEA5/OGA at approximately 130 kDa. The expected band size for MGEA5/OGA is at 103 kDa.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.