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Anti-CD36 Antibody (Clone#OTI3F4)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF FCM

MA01189

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Western blot analysis of anti-CD36 antibody (MA01189). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human placenta tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD36 antigen affinity purified monoclonal antibody (MA01189) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.

    all(9)
  • Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01189-1, Dilution 1:50)

    all(9)
  • Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01189-1, Dilution 1:50)

    all(9)
  • Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01189-1) (1:500)

    all(9)
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01189-1) (1:500)

    all(9)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01189-1) (1:500)

    all(9)
  • IHC analysis of CD36 using anti-CD36 antibody (MA01189).
    CD36 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with mouse anti-CD36 Antibody (MA01189) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

    all(9)
  • Anti-CD36 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD36.

    all(9)
  • HEK293T cells transfected with either overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-CD36 antibody, and then analyzed by flow cytometry (1:100).

    all(9)

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产品简介>

产品名称
Anti-CD36 Antibody (Clone#OTI3F4)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD36; CHDS7; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; PAS 4; PAS IV; PASIV; Platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; Thrombospondin receptor
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
CD36
克隆号
OTI3F4
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human CD36 (NP_000063) produced in HEK293T cell.
计算分子量
53 kDa
实际分子量
88 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Uniprot ID
P16671  
文献引用格式
CD36 Antibody (Clone#OTI3F4) (Boster Biological Technology, Wuhan, China. Catalog#MA01189)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
CD36 molecule
蛋白名全称
Platelet glycoprotein 4
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow Cytometry(FCM):1:100

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    Anti-CD36 Antibody (Clone#OTI3F4)

    筛选器: All WB IHC ICC/IF FCM

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