Anti-GDNF Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PA1465

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GDNF using anti-GDNF antibody (PA1465).
Lane 1: recombinant human GDNF protein 10 ng,
Lane 2: recombinant human GDNF protein 5 ng,
Lane 3: recombinant human GDNF protein 2.5 ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody (PA1465) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GDNF at approximately 17 kDa.
all(4)
IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-GDNF Antibody (PA1465) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-GDNF Antibody (PA1465) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GDNF Antibody (PA1465) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-GDNF Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GDNF; HFB1 GDNF; hGDNF; ATF; ATF1; ATF2; HFB1-GDNF; HSCR3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

GDNF

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human GDNF, different from the related mouse sequence by three amino acids and rat sequence by two amino acids.

计算分子量

24 kDa

实际分子量

17 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.

Uniprot ID

P39905

RRID

AB_3082680

文献引用格式

GDNF Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1465)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

glial cell derived neurotrophic factor

蛋白名全称

Glial cell line-derived neurotrophic factor(hGDNF)

实验方案>

实验步骤

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