Anti-Angiopoietin-1/ANGPT1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0092

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Angiopoietin-1/ANGPT1 using anti-Angiopoietin-1/ANGPT1 antibody (PB0092).
Lane 1: recombinant Human Angiopoietin1 Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Angiopoietin-1/ANGPT1 antigen affinity purified polyclonal antibody (PB0092) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Angiopoietin-1/ANGPT1 at approximately 39 kDa.
all(2)
Western blot analysis of Angiopoietin-1/ANGPT1 using anti-Angiopoietin-1/ANGPT1 antibody (PB0092). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: COLO320 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: HEPG2 whole cell lysates,
Lane 6: 293T whole cell lysates,
Lane 7: SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Angiopoietin-1/ANGPT1 antigen affinity purified polyclonal antibody (PB0092) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Angiopoietin-1/ANGPT1 at approximately 57 kDa/70 kDa. The expected band size for Angiopoietin-1/ANGPT1 is at 58 kDa.
all(2)

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产品简介>

产品名称

Anti-Angiopoietin-1/ANGPT1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AGP1; AGPT; ANG 1; ANG1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

ANGPT1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Angiopoietin 1 recombinant protein (Position: H16-N350). Human Angiopoietin 1 shares 96% amino acid (aa) sequence with both mouse and rat Angiopoietin 1.

计算分子量

58 kDa

实际分子量

57 kDa/70 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Angiopoietin 1 is mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Uniprot ID

Q15389

文献引用格式

Angiopoietin-1/ANGPT1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0092)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

angiopoietin 1

蛋白名全称

Angiopoietin-1

实验方案>

实验步骤

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