Anti-Collagen Type III/COL3A1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

PB0125

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (PB0125).
Lane 1: recombinant human collagen Ⅲ protein.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type III/COL3A1 antigen affinity purified polyclonal antibody (PB0125) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type III/COL3A1 at approximately 150 kDa-200 kDa.
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Figure 2. Western blot analysis of Collagen Type III/COL3A1 using anti-Collagen Type III/COL3A1 antibody (PB0125). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-4 whole cell lysates,
Lane 2: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type III/COL3A1 antigen affinity purified polyclonal antibody (PB0125) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type III/COL3A1 at approximately 150 kDa-200 kDa. The expected band size for Collagen Type III/COL3A1 is at 139 kDa.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Collagen Type III/COL3A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

COL3A1; Collagen alpha 1(III) chain; Collagen Type III; EDS4A

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

COL3A1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

计算分子量

139 kDa

实际分子量

150 kDa-200 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

COL3A1, also called EDS4A or Collagen alpha-1(III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1(III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12

Uniprot ID

P02461

文献引用格式

Collagen Type III/COL3A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0125)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

collagen, type III, alpha 1

蛋白名全称

Collagen alpha-1(III) chain

实验方案>

实验步骤

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