Anti-PAPPA Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0340

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PAPPA using anti-PAPPA antibody (PB0340). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human Placenta tissue lysates,
Lane 2: HT1080 whole cell lysates,
Lane 3: SKOV whole cell lysates,
Lane 4: 22RV1 whole cell lysates,
Lane 5: SW620 whole cell lysates,
Lane 6: MM231 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAPPA antigen affinity purified polyclonal antibody (PB0340) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PAPPA at approximately 181 kDa. The expected band size for PAPPA is at 181 kDa.
all(2)
IHC analysis of PAPPA using anti-PAPPA antibody (PB0340).
PAPPA was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PAPPA Antibody (PB0340) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-PAPPA Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

pappalysin 1; ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A1; PAPPA; IGFBP 4ase; PAPP A; PAPPA1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

PAPPA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A.

计算分子量

181 kDa

实际分子量

181 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Pappalysin-1, also known as DIPLA1, is a protein that in humans is encoded by the PAPPA gene. It is mapped to 9q33.1. PAPPA is found in the ovarian follicles, follicular fluid, luteal cells, and fallopian tubes of nonpregnant women and in the seminal vesicles and seminal fluid of males. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. It has been found that circulating PAPPA is a disulfide-bridged complex with proMBP in which the subunits of the constituents are present in a 1:1 molar ratio.

Uniprot ID

Q13219

文献引用格式

PAPPA Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0340)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

pregnancy-associated plasma protein A, pappalysin 1

蛋白名全称

Pappalysin-1

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实验步骤

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