Anti-ABCG5 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0381

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ABCG5 using anti-ABCG5 antibody (PB0381). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: PANC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (PB0381) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCG5 at approximately 73 kDa. The expected band size for ABCG5 is at 73 kDa.
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产品名称

Anti-ABCG5 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ABCG5; Sterolin 1; STSL

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

ABCG5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5 different from the related mouse and rat sequences by two amino acids.

计算分子量

73 kDa

实际分子量

73 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb. The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8. The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.

Uniprot ID

Q9H222

文献引用格式

ABCG5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0381)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATP-binding cassette, sub-family G (WHITE), member 5

蛋白名全称

ATP-binding cassette sub-family G member 5

实验方案>

实验步骤

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