Anti-TRPV5 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0549

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TRPV5 using anti-TRPV5 antibody (PB0549). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Pancreas tissue lysates,
Lane 2: Rat Lung tissue lysates,
Lane 3: Rat Intestine tissue lysates,
Lane 4: SW620 whole cell lysates,
Lane 5: COLO320 whole cell lysates,
Lane 6: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (PB0549) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRPV5 at approximately 83 kDa. The expected band size for TRPV5 is at 83 kDa.
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产品简介>

产品名称

Anti-TRPV5 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Calcium transport protein 2; CAT2; ECaC; ECAC1; Epithelial calcium channel 1; Osm 9 like TRP channel 3; OTRPC3; TRPV5

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

TRPV5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TRPV5 different from the related mouse and rat sequences by one amino acid.

计算分子量

83 kDa

实际分子量

83 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Transient receptor potential cation channel subfamily V member 5 is a protein that in humans is encoded by the TRPV5 gene. This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. And this protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. In addition, TRPV5 is mainly expressed in kidney epithelial cells, where it plays an important role in the reabsorption of Ca2+. Genetic deletion of TRPV5 in mice leads to Ca2+ loss in the urine, and consequential hyperparathyroidism, and bone loss.

Uniprot ID

Q9NQA5

文献引用格式

TRPV5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0549)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transient receptor potential cation channel, subfamily V, member 5

蛋白名全称

Transient receptor potential cation channel subfamily V member 5

实验方案>

实验步骤

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