Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of MIP-2/CXCL2 using anti-MIP-2/CXCL2 antibody (PB0900).
Lane 1: recombinant rat CXCL2 protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIP-2/CXCL2 antigen affinity purified polyclonal antibody (PB0900) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIP-2/CXCL2 at approximately 11 kDa.
Western blot analysis of MIP-2/CXCL2 using anti-MIP-2/CXCL2 antibody (PB0900).
Lane 1: recombinant rat CXCL2 protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIP-2/CXCL2 antigen affinity purified polyclonal antibody (PB0900) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIP-2/CXCL2 at approximately 11 kDa.