Anti-ACTN3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM

PB10026

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ACTN3 using anti-ACTN3 antibody (PB10026). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HT1080 whole cell lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat skeletal muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACTN3 antigen affinity purified polyclonal antibody (PB10026) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACTN3 at approximately 103 kDa. The expected band size for ACTN3 is at 103 kDa.
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IHC analysis of ACTN3 using anti-ACTN3 antibody (PB10026).
ACTN3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN3 Antibody (PB10026) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ACTN3 using anti-ACTN3 antibody (PB10026).
ACTN3 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN3 Antibody (PB10026) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ACTN3 using anti-ACTN3 antibody (PB10026).
ACTN3 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACTN3 Antibody (PB10026) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of ACTN3 using anti-ACTN3 antibody (PB10026).
ACTN3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. The tissue section was incubated with rabbit anti-ACTN3 Antibody (PB10026) at a dilution of 1:100. FITC Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) Secondary Antibody (green) (Catalog # BA1105) was used as secondary antibody.
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Flow Cytometry analysis of WISH cells using anti-ACTN3 antibody (PB10026).
Overlay histogram showing WISH cells stained with PB10026 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACTN3 Antibody (PB10026) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ACTN3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

actinin; alpha 3; ACTN3; Alpha actinin 3; F actin cross linking protein

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM

基因名称

ACTN3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

计算分子量

103 kDa

实际分子量

103 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Uniprot ID

Q08043

文献引用格式

ACTN3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB10026)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

actinin alpha 3 (gene/pseudogene)

蛋白名全称

Alpha-actinin-3

实验方案>

实验步骤

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