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Anti-AGO2 Antibody

Rabbit polyclonal antibody

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筛选器: All WB

PB1030

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of AGO2 using anti-AGO2 antibody (PB1030). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Jurkat whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human K562 whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates,
    Lane 5: rat lung tissue lysates,
    Lane 6: rat pancrease tissue lysates,
    Lane 7: mouse pancrease tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGO2 antigen affinity purified polyclonal antibody (PB1030) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGO2 at approximately 97 kDa. The expected band size for AGO2 is at 97 kDa.

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产品名称
Anti-AGO2 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
AGO2; Argonaute2; eIF 2C 2; eIF2C 2; EIF2C2; hAgo2; PAZ Piwi domain protein; PPD; Protein argonaute 2; Protein slicer; Q10
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB
基因名称
AGO2
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human Ago2/ eIF2C2 identical to the related mouse and rat sequences.
计算分子量
97 kDa
实际分子量
97 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Protein argonaute-2, also known as AGO2, is a protein that in humans is encoded by the EIF2C2 gene. This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Uniprot ID
Q9UKV8  
文献引用格式
AGO2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1030)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
argonaute 2, RISC catalytic component
蛋白名全称
Protein argonaute-2
推荐稀释比
Western blot (WB):1:500-2000

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    Anti-AGO2 Antibody

    筛选器: All WB

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