Anti-ABCE1 Antibody

筛选器： All WB ICC/IF IP FCM

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  Western blot analysis of ABCE1 using anti-ABCE1 antibody (PB1072). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human A431 whole cell lysates,
  Lane 3: human U2OS whole cell lysates,
  Lane 4: human Jurkat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: mouse brain tissue lysates,
  Lane 7: mouse 4T1 whole cell lysates,
  Lane 8: mouse NIN/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (PB1072) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.

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  IF analysis of ABCE1 using anti-ABCE1 antibody (PB1072).
  ABCE1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABCE1 Antibody (PB1072) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  IP analysis of ABCE1 using anti-ABCE1 antibody (PB1072) in A431 whole cell lysate.
  Western blot analysis of ABCE1 using anti- ABCE1 antibody (PB1072).
  Lane 1: A431 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- ABCE1 antibody in A431 whole cell lysate,
  Lane 3: anti- ABCE1 antibody (2μg) + A431 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ABCE1 antigen affinity purified polyclonal antibody (PB1072) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Heavy Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.

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  Flow Cytometry analysis of U251 cells using anti-ABCE1 antibody (PB1072).
  Overlay histogram showing U251 cells stained with PB1072 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody (PB1072) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of ABCE1 using anti-ABCE1 antibody (PB1072). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human A431 whole cell lysates,
  Lane 3: human U2OS whole cell lysates,
  Lane 4: human Jurkat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: mouse brain tissue lysates,
  Lane 7: mouse 4T1 whole cell lysates,
  Lane 8: mouse NIN/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (PB1072) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.

• 

  IF analysis of ABCE1 using anti-ABCE1 antibody (PB1072).
  ABCE1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABCE1 Antibody (PB1072) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  IP analysis of ABCE1 using anti-ABCE1 antibody (PB1072) in A431 whole cell lysate.
  Western blot analysis of ABCE1 using anti- ABCE1 antibody (PB1072).
  Lane 1: A431 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- ABCE1 antibody in A431 whole cell lysate,
  Lane 3: anti- ABCE1 antibody (2μg) + A431 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ABCE1 antigen affinity purified polyclonal antibody (PB1072) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Heavy Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.

• 

  Flow Cytometry analysis of U251 cells using anti-ABCE1 antibody (PB1072).
  Overlay histogram showing U251 cells stained with PB1072 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody (PB1072) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.