Anti-SYN2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB1153

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SYN2 using anti-SYN2 antibody (PB1153). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SYN2 antigA03957-Aen affinity purified polyclonal antibody (PB1153) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SYN2 at approximately 55,80 kDa. The expected band size for SYN2 is at 63 kDa.
all(5)
IHC analysis of SYN2 using anti-SYN2 antibody (PB1153).
SYN2 was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-SYN2 Antibody (PB1153) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of SYN2 using anti-SYN2 antibody (PB1153).
SYN2 was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-SYN2 Antibody (PB1153) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of SYN2 using anti-SYN2 antibody (PB1153).
SYN2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SYN2 Antibody (PB1153) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IF analysis of SYN2 using anti-SYN2 antibody (PB1153) and anti-Alpha Tubulin antibody (M03989-3).
SYN2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SYN2 Antibody (PB1153) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
all(5)

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产品简介>

产品名称

Anti-SYN2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

SYN2; synapsin II; SYNII; SYNIIa; SYNIIb

产品类型

Polyclonal

检验物种

human, mouse, rat, pig

应用范围

WB, IHC, ICC/IF

基因名称

SYN2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Synapsin II recombinant protein (Position: A497-D582). Human Synapsin II shares 93.1% and 89.7% amino acid (aa) sequence identity with mouse and rat Synapsin II, respectively.

计算分子量

63 kDa

实际分子量

55,80 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Synapsin II is the collective name for synapsin IIa and synapsin IIb, two nearly identical phosphoproteins in the synapsin family that in humans are encoded by the SYN2 gene. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Polymorphisms in this gene are associated with abnormal presynaptic function and related neuronal disorders, including autism, epilepsy, bipolar disorder and schizophrenia. Alternative splicing of this gene results in multiple transcript variants. The tissue inhibitor of metalloproteinase 4 gene is located within an intron of this gene and is transcribed in the opposite direction.

Uniprot ID

Q92777

文献引用格式

SYN2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1153)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

synapsin II

蛋白名全称

Synapsin-2

实验方案>

实验步骤

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