Anti-BDNF Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9075

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of BDNF using anti-BDNF antibody (PB9075).
Lane 1: recombinant human BDNF protein 5 ng,
Lane 2: recombinant human BDNF protein 2.5 ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDNF antigen affinity purified polyclonal antibody (PB9075) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDNF at approximately 14 kDa.
all(4)
IHC analysis of BDNF using anti-BDNF antibody (PB9075).
BDNF was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-BDNF Antibody (PB9075) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of BDNF using anti-BDNF antibody (PB9075).
BDNF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-BDNF Antibody (PB9075) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of BDNF using anti-BDNF antibody (PB9075).
BDNF was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-BDNF Antibody (PB9075) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-BDNF Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Abrineurin; BDNF

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

BDNF

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.

计算分子量

28 kDa

实际分子量

14 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Uniprot ID

P21237

RRID

AB_3081957

文献引用格式

BDNF Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9075)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

brain-derived neurotrophic factor

蛋白名全称

Brain-derived neurotrophic factor

实验方案>

实验步骤

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