Figure 1. Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
Lane 1: recombinant human AAMP protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa.
Figure 2. Western blot analysis of AAMP using anti-AAMP antibody (PB9123). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: HEPG2 whole cell lysates,
Lane 4: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa. The expected band size for AAMP is at 47 kDa.
Figure 3. IHC analysis of AAMP using anti-AAMP antibody (PB9123).
AAMP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AAMP Antibody (PB9123) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. Flow cytometry analysis of K562 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(4) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
Lane 1: recombinant human AAMP protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa.
Figure 2. Western blot analysis of AAMP using anti-AAMP antibody (PB9123). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: HEPG2 whole cell lysates,
Lane 4: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa. The expected band size for AAMP is at 47 kDa.
Figure 3. IHC analysis of AAMP using anti-AAMP antibody (PB9123).
AAMP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AAMP Antibody (PB9123) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. Flow cytometry analysis of K562 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
Lane 1: recombinant human AAMP protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa.
Figure 2. Western blot analysis of AAMP using anti-AAMP antibody (PB9123). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3: HEPG2 whole cell lysates,
Lane 4: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AAMP at approximately 47 kDa. The expected band size for AAMP is at 47 kDa.
Figure 3. IHC analysis of AAMP using anti-AAMP antibody (PB9123).
AAMP was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AAMP Antibody (PB9123) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. Flow cytometry analysis of K562 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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