Anti-IkB Beta/NFKBIB Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9292

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IkB Beta/NFKBIB using anti-IkB Beta/NFKBIB antibody (PB9292). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IkB Beta/NFKBIB antigen affinity purified polyclonal antibody (PB9292) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IkB Beta/NFKBIB at approximately 45-48 kDa. The expected band size for IkB Beta/NFKBIB is at 38 kDa.
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IHC analysis of IkB Beta/NFKBIB using anti-IkB Beta/NFKBIB antibody (PB9292).
IkB Beta/NFKBIB was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-IkB Beta/NFKBIB Antibody (PB9292) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-IkB Beta/NFKBIB Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

I kappa B beta; IkappaBbeta; IkB B; IkB beta; IKBB; NF kappa B inhibitor beta; NF kappa BIB; NFKBIB; TR interacting protein 9; TRIP 9; TRIP9

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NFKBIB

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively.

计算分子量

38 kDa

实际分子量

45-48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. This gene is mapped to 19q13.2. It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response. NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha. Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.

Uniprot ID

Q15653

文献引用格式

IkB Beta/NFKBIB Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9292)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta

蛋白名全称

NF-kappa-B inhibitor beta

实验方案>

实验步骤

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