Anti-PARK2/Parkin/PRKN Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB9307

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PARK2/Parkin/PRKN using anti-PARK2/Parkin/PRKN antibody (PB9307). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U87 whole cell lysates,
Lane 2: Mouse Brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PARK2/Parkin/PRKN antigen affinity purified polyclonal antibody (PB9307) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PARK2/Parkin/PRKN at approximately 52,66 kDa. The expected band size for PARK2/Parkin/PRKN is at 52 kDa.
all(5)
IHC analysis of PARK2/Parkin/PRKN using anti-PARK2/Parkin/PRKN antibody (PB9307).
PARK2/Parkin/PRKN was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PARK2/Parkin/PRKN Antibody (PB9307) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of PARK2/Parkin/PRKN using anti-PARK2/Parkin/PRKN antibody (PB9307).
PARK2/Parkin/PRKN was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PARK2/Parkin/PRKN Antibody (PB9307) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of PARK2/Parkin/PRKN using anti-PARK2/Parkin/PRKN antibody (PB9307).
PARK2/Parkin/PRKN was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PARK2/Parkin/PRKN Antibody (PB9307) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Parkin using anti-Parkin antibody (PB9307).
Parkin was detected in immunocytochemical section of NEURO-2α Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Parkin Antibody (PB9307) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PARK2/Parkin/PRKN Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AR JP; LPRS2; PARK2; PARK2/Parkin; Parkin; Parkinson disease protein 2; PDJ; PRKN

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

PRKN

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.

计算分子量

52 kDa

实际分子量

52,66 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.

Uniprot ID

O60260

RRID

AB_3082058

文献引用格式

PARK2/Parkin/PRKN Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9307)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

parkin RBR E3 ubiquitin protein ligase

蛋白名全称

E3 ubiquitin-protein ligase parkin

实验方案>

实验步骤

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