Anti-PKM2/PKM Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB9379

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PKM2/PKM using anti-PKM2/PKM antibody (PB9379). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: MDA-B-453 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: U87 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: L6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKM2/PKM antigen affinity purified polyclonal antibody (PB9379) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKM2/PKM at approximately 58 kDa. The expected band size for PKM2/PKM is at 58 kDa.
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IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody (PB9379).
PKM2/PKM was detected in a paraffin-embedded section of rat skeletal muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKM2/PKM Antibody (PB9379) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody (PB9379).
PKM2/PKM was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKM2/PKM Antibody (PB9379) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody (PB9379).
PKM2/PKM was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKM2/PKM Antibody (PB9379) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of PKM2/PKM using anti-PKM2/PKM antibody (PB9379).
PKM2/PKM was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKM2/PKM Antibody (PB9379) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of PKM2/PKM using anti-PKM2/PKM antibody (PB9379).
PKM2/PKM was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-PKM2/PKM Antibody (PB9379) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-PKM2/PKM Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CTHBP; OIP 3; OIP3; Opa interacting protein 3; p58; PK2; PK3; PKM; PKM2; PK-M2; PKM2 pyruvate kinase; muscle; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; TCB; THBP1; Tumor M2 PK

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

PKM

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

计算分子量

58 kDa

实际分子量

58 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

Uniprot ID

P14618

RRID

AB_3082092

文献引用格式

PKM2/PKM Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9379)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

pyruvate kinase, muscle

蛋白名全称

Pyruvate kinase PKM

实验方案>

实验步骤

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