Anti-NKCC2/SLC12A1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

PB9392

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NKCC2/SLC12A1 using anti-NKCC2/SLC12A1 antibody (PB9392). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey kidney tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NKCC2/SLC12A1 antigen affinity purified polyclonal antibody (PB9392) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NKCC2/SLC12A1 at approximately 150-290 kDa. The expected band size for NKCC2/SLC12A1 is at 121 kDa.
all(6)
IHC analysis of NKCC2/SLC12A1 using anti-NKCC2/SLC12A1 antibody (PB9392).
NKCC2/SLC12A1 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NKCC2/SLC12A1 Antibody (PB9392) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of NKCC2/SLC12A1 using anti-NKCC2/SLC12A1 antibody (PB9392).
NKCC2/SLC12A1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NKCC2/SLC12A1 Antibody (PB9392) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of NKCC2/SLC12A1 using anti-NKCC2/SLC12A1 antibody (PB9392).
NKCC2/SLC12A1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NKCC2/SLC12A1 Antibody (PB9392) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of NKCC2/SLC12A1 using anti-NKCC2/SLC12A1 antibody (PB9392).
NKCC2/SLC12A1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-NKCC2/SLC12A1 Antibody (PB9392) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
IF analysis of NKCC2/SLC12A1 using anti-NKCC2/SLC12A1 antibody (PB9392).
NKCC2/SLC12A1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-NKCC2/SLC12A1 Antibody (PB9392) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NKCC2/SLC12A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSC1; NKCC2; SLC12A1

产品类型

Polyclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, IHC, IF

基因名称

SLC12A1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.

计算分子量

121 kDa

实际分子量

150-290 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Solute carrier family 12 (sodium/potassium/chloride transporters), member 1, also called NKCC2 is specifically found in cells of the thick ascending limb of the loop of Henle in nephrons, the basic functional units of the kidney. This gene is mapped to 15q21.1. This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. This gene plays a vital role in the regulation of ionic balance and cell volume.

Uniprot ID

Q13621

RRID

AB_3082097

文献引用格式

NKCC2/SLC12A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9392)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

solute carrier family 12 (sodium/potassium/chloride transporters), member 1

蛋白名全称

Solute carrier family 12 member 1

实验方案>

实验步骤

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