Anti-VCAN Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9453

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VCAN using anti-VCAN antibody (PB9453). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: HEPA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VCAN antigen affinity purified polyclonal antibody (PB9453) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VCAN at approximately 373 kDa. The expected band size for VCAN is at 373 kDa.
all(2)
IHC analysis of VCAN using anti-VCAN antibody (PB9453).
VCAN was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VCAN Antibody (PB9453) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-VCAN Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

versican; CSPG2; ERVR; GHAP; PG-M; WGN; WGN1; VCAN

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

VCAN

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids.

计算分子量

373 kDa

实际分子量

373 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Uniprot ID

P13611

RRID

AB_3082121

文献引用格式

VCAN Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9453)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

versican

蛋白名全称

Versican core protein

实验方案>

实验步骤

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