Anti-Aquaporin 4/AQP4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9475

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Aquaporin 4/AQP4 antibody (PB9475). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Aquaporin 4/AQP4 antigen affinity purified polyclonal antibody (PB9475) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Aquaporin 4/AQP4 at approximately 37 kDa. The expected band size for Aquaporin 4/AQP4 is at 35 kDa.
all(4)
IHC analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (PB9475).
Aquaporin 4/AQP4 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (PB9475).
Aquaporin 4/AQP4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (PB9475).
Aquaporin 4/AQP4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品名称

Anti-Aquaporin 4/AQP4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AQP 4; AQP4; aquaporin 4; HMIWC2; MIWC; NMO-IgG; WCH4

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

AQP4

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

计算分子量

35 kDa

实际分子量

35 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

Uniprot ID

P55087

RRID

AB_3082133

文献引用格式

Aquaporin 4/AQP4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9475)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aquaporin 4

蛋白名全称

Aquaporin-4

实验方案>

实验步骤

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