Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse Lung tissue lysates,
Lane 2: Mouse Intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9609) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of A431 cells using anti-Lipocalin 2 antibody (PB9609).
Overlay histogram showing A431 cells stained with PB9609 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lipocalin 2 Antibody (PB9609, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| ELISA(Cap) | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse Lung tissue lysates,
Lane 2: Mouse Intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9609) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of A431 cells using anti-Lipocalin 2 antibody (PB9609).
Overlay histogram showing A431 cells stained with PB9609 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lipocalin 2 Antibody (PB9609, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse Lung tissue lysates,
Lane 2: Mouse Intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9609) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9609).
Lipocalin-2/NGAL/LCN2 was detected in frozen section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 Antibody (PB9609) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of A431 cells using anti-Lipocalin 2 antibody (PB9609).
Overlay histogram showing A431 cells stained with PB9609 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lipocalin 2 Antibody (PB9609, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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