Anti-IGFBP3 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC ELISA

PB9710

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (PB9710). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: human U-87MG whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (PB9710) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.
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Figure 2. IHC analysis of IGFBP3 using anti-IGFBP3 antibody (PB9710).
IGFBP3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGFBP3 Antibody (PB9710) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IGFBP3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BP 53; IBP 3; IBP3; IGF binding protein 3; IGFBP 3; IGFBP3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

IGFBP3

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human IGFBP-3, identical to the related mouse and rat sequences.

计算分子量

32 kDa

实际分子量

32 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Uniprot ID

P17936

RRID

AB_3082232

文献引用格式

IGFBP3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9710)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

insulin-like growth factor binding protein 3

蛋白名全称

Insulin-like growth factor-binding protein 3

实验方案>

实验步骤

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