Anti-HIF-1 alpha/HIF1A Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00013-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HIF-1 alpha/HIF1A using anti-HIF-1 alpha/HIF1A antibody (A00013-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HIF-1 alpha/HIF1A antigen affinity purified polyclonal antibody (A00013-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa. The expected band size for HIF-1 alpha/HIF1A is at 93 kDa.
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产品名称

Anti-HIF-1 alpha/HIF1A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARNT interacting protein; bHLHe78; HIF 1 alpha; HIF 1alpha; HIF1; HIF-1; HIF1 ALPHA; HIF1A; HIF-1α; Member of PAS protein 1; MOP1; PASD8

产品类型

Polyclonal

检验物种

human

应用范围

WB, ELISA

基因名称

HIF1A

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human HIF-1 alpha/HIF1A recombinant protein (Position: H197-R718).

计算分子量

93 kDa

实际分子量

120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

HIF-1α (Hypoxia-inducible factor 1α,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Uniprot ID

Q16665

RRID

AB_3080879

文献引用格式

HIF-1 alpha/HIF1A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00013-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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