Anti-SIRT1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A00018-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SIRT1 using anti-SIRT1 antibody (A00018-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (A00018-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.
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Western blot analysis of SIRT1 using anti-SIRT1 antibody (A00018-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (A00018-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.
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IF analysis of SIRT1 using anti-SIRT1 antibody (A00018-1) and anti-Beta Tubulin antibody (M01857-3).
SIRT1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SIRT1 Antibody (A00018-1) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
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产品简介>

产品名称

Anti-SIRT1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

hSIR2; hSIRT1; SIR2 like protein 1; SIR2L1; SIRT1

产品类型

Polyclonal

检验物种

human

应用范围

WB, ICC/IF, ELISA

基因名称

SIRT1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human SIRT1 recombinant protein (Position: Y142-H533). Human SIRT1 shares 96.9% and 99.4% amino acid (aa) sequence identity with mouse and rat SIRT1, respectively.

计算分子量

82 kDa

实际分子量

110-120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

Uniprot ID

Q96EB6

文献引用格式

SIRT1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00018-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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