Anti-NLRP3 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of NLRP3 using anti-NLRP3 antibody (A00034-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HELA whole cell lysates,
  Lane 2: human U-87MG whole cell lysates,
  Lane 3: rat spleen tissue lysates,
  Lane 4: Rat PC-12 whole cell lysates,
  Lane 5: mouse thymus tissue lysates,
  Lane 6: mouse lung tissue lysates,
  Lane 7: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (A00034-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NLRP3 at approximately 118 kDa. The expected band size for NLRP3 is at 118 kDa.

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  Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (A00034-2).
  Overlay histogram showing THP-1 cells stained with A00034-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (A00034-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of NLRP3 using anti-NLRP3 antibody (A00034-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HELA whole cell lysates,
  Lane 2: human U-87MG whole cell lysates,
  Lane 3: rat spleen tissue lysates,
  Lane 4: Rat PC-12 whole cell lysates,
  Lane 5: mouse thymus tissue lysates,
  Lane 6: mouse lung tissue lysates,
  Lane 7: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (A00034-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NLRP3 at approximately 118 kDa. The expected band size for NLRP3 is at 118 kDa.

• 

  Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (A00034-2).
  Overlay histogram showing THP-1 cells stained with A00034-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (A00034-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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