Anti-NLRP3 Antibody

筛选器： All WB IHC FCM

• 

  Western blot analysis of NLRP3 using anti-NLRP3 antibody (BA3677). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human THP-1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (BA3677) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118 kDa.

• 

  Western blot analysis of NLRP3 using anti-NLRP3 antibody (BA3677). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat thymus tissue lysates,
  Lane 2: mouse thymus tissue lysates,
  Lane 3: mouse RAW264.7 whole cell lysates,
  Lane 4: mouse J774A.1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (BA3677) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118 kDa.

• 

  IHC analysis of NLRP3 using anti-NLRP3 antibody (BA3677).
  NLRP3 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-NLRP3 Antibody (BA3677) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (BA3677).
  Overlay histogram showing THP-1 cells stained with BA3677 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (BA3677) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of NLRP3 using anti-NLRP3 antibody (BA3677). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human THP-1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (BA3677) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118 kDa.

• 

  Western blot analysis of NLRP3 using anti-NLRP3 antibody (BA3677). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat thymus tissue lysates,
  Lane 2: mouse thymus tissue lysates,
  Lane 3: mouse RAW264.7 whole cell lysates,
  Lane 4: mouse J774A.1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (BA3677) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118 kDa.

• 

  IHC analysis of NLRP3 using anti-NLRP3 antibody (BA3677).
  NLRP3 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-NLRP3 Antibody (BA3677) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (BA3677).
  Overlay histogram showing THP-1 cells stained with BA3677 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (BA3677) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.