Anti-Caspase 9/CASP9 Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (A00080-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 9/CASP9 antigen affinity purified polyclonal antibody (A00080-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 9/CASP9 at approximately 46 kDa. The expected band size for Caspase 9/CASP9 is at 46 kDa.

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  Figure 2. IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (A00080-5).
  Caspase 9/CASP9 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 9/CASP9 Antibody (A00080-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IF analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (A00080-5).
  Caspase 9/CASP9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Caspase 9/CASP9 Antibody (A00080-5) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 4. Flow Cytometry analysis of U937 cells using anti-Caspase 9/CASP9 antibody (A00080-5).
  Overlay histogram showing U937 cells stained with A00080-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 9/CASP9 Antibody (A00080-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (A00080-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 9/CASP9 antigen affinity purified polyclonal antibody (A00080-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 9/CASP9 at approximately 46 kDa. The expected band size for Caspase 9/CASP9 is at 46 kDa.

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  Figure 2. IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (A00080-5).
  Caspase 9/CASP9 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Caspase 9/CASP9 Antibody (A00080-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IF analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (A00080-5).
  Caspase 9/CASP9 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Caspase 9/CASP9 Antibody (A00080-5) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 4. Flow Cytometry analysis of U937 cells using anti-Caspase 9/CASP9 antibody (A00080-5).
  Overlay histogram showing U937 cells stained with A00080-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 9/CASP9 Antibody (A00080-5) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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