Anti-Caspase 9/CASP9 Antibody (Clone#GEO-3)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP

BM4619

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of anti-Caspase 9/CASP9 antibody (BM4619). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 9/CASP9 antigen affinity purified monoclonal antibody (BM4619) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 9/CASP9 at approximately 46 kDa. The expected band size for Caspase 9/CASP9 is at 46 kDa.
all(4)
Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-9 Antibody.
all(4)
Immunofluorescent analysis of HeLa cells treated with staurosporine, using Caspase-9 Antibody.
all(4)
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Caspase 9/CASP9 Antibody (Clone#GEO-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

APAF 3; APAF3; Apoptotic protease Mch 6; CASP 9; CASP9; CASP-9; Caspase 9; CASPASE 9c; Caspase9; Caspase-9; ICE LAP6; ICE like apoptotic protease 6; MCH6

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF, IP

基因名称

CASP9

克隆号

GEO-3

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Caspase-9

计算分子量

46 kDa

实际分子量

46 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Uniprot ID

P55211

文献引用格式

Caspase 9/CASP9 Antibody (Clone#GEO-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4619)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Caspase-9

实验方案>

实验步骤

引用文献(0)>

清除