Western blot analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (A00084-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse RAW264.7(-LPS) whole cell lysates,
Lane 3: mouse RAW264.7(+LPS) whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 antigen affinity purified polyclonal antibody (A00084-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX2/Cyclooxygenase 2/PTGS2 at approximately 75 kDa. The expected band size for COX2/Cyclooxygenase 2/PTGS2 is at 69 kDa.
IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2) .
PTGS2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-PTGS2 Antibody (A00084-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2) .
PTGS2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. The tissue section was incubated with rabbit anti-PTGS2 Antibody (A00084-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow cytometry analysis of CACO-2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
all(6) 
Flow cytometry analysis of HEPA1-6 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
all(6) Western blot analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (A00084-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse RAW264.7(-LPS) whole cell lysates,
Lane 3: mouse RAW264.7(+LPS) whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 antigen affinity purified polyclonal antibody (A00084-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX2/Cyclooxygenase 2/PTGS2 at approximately 75 kDa. The expected band size for COX2/Cyclooxygenase 2/PTGS2 is at 69 kDa.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (A00084-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse RAW264.7(-LPS) whole cell lysates,
Lane 3: mouse RAW264.7(+LPS) whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 antigen affinity purified polyclonal antibody (A00084-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX2/Cyclooxygenase 2/PTGS2 at approximately 75 kDa. The expected band size for COX2/Cyclooxygenase 2/PTGS2 is at 69 kDa.
IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2) .
PTGS2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-PTGS2 Antibody (A00084-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2) .
PTGS2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. The tissue section was incubated with rabbit anti-PTGS2 Antibody (A00084-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow cytometry analysis of CACO-2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Flow cytometry analysis of HEPA1-6 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Western blot analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (A00084-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse RAW264.7(-LPS) whole cell lysates,
Lane 3: mouse RAW264.7(+LPS) whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 antigen affinity purified polyclonal antibody (A00084-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX2/Cyclooxygenase 2/PTGS2 at approximately 75 kDa. The expected band size for COX2/Cyclooxygenase 2/PTGS2 is at 69 kDa.
IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2) .
PTGS2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-PTGS2 Antibody (A00084-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2) .
PTGS2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. The tissue section was incubated with rabbit anti-PTGS2 Antibody (A00084-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Flow cytometry analysis of CACO-2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Flow cytometry analysis of HEPA1-6 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
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