Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

BA3708

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (BA3708). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse RAW264.7(+lps） whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 antigen affinity purified polyclonal antibody (BA3708) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX2/Cyclooxygenase 2/PTGS2 at approximately 75 kDa. The expected band size for COX2/Cyclooxygenase 2/PTGS2 is at 69 kDa.
all(6)
IHC analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (BA3708).
COX2/Cyclooxygenase 2/PTGS2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 Antibody (BA3708) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (BA3708).
COX2/Cyclooxygenase 2/PTGS2 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 Antibody (BA3708) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (BA3708).
COX2/Cyclooxygenase 2/PTGS2 was detected in a paraffin-embedded section of human differentiated adenocarcinoma in the appendix-1 tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 Antibody (BA3708) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (BA3708).
COX2/Cyclooxygenase 2/PTGS2 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 Antibody (BA3708) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of COX2/Cyclooxygenase 2/PTGS2 using anti-COX2/Cyclooxygenase 2/PTGS2 antibody (BA3708).
COX2/Cyclooxygenase 2/PTGS2 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX2/Cyclooxygenase 2/PTGS2 Antibody (BA3708) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

COX 2; COX2; COX2/ Cyclooxygenase 2; COX2/ Cyclooxygenase 2/ PTGS2; Cyclooxygenase 2; GRIPGHS; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PHS 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; PTGS2

产品类型

Polyclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

PTGS2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human COX2.

计算分子量

69 kDa

实际分子量

75 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Cyclooxygenase(Cox) is the key enzyme in conversion of arachidonic acid to PGs, and two isoforms, Cox-1 and Cox-2, have been identified. Cox-2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine Cox-2 gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis. This gene is localized to sites associated with retinal blood vessels, and plays an important role in blood vessel formation in the retina. And the glucocorticoid receptor suppression of COX-2 is also crucial for curtailing lethal immune activation, and suggest new therapeutic approaches for regulation of T-cell-mediated inflammatory diseases.

Uniprot ID

P35354

文献引用格式

COX2/Cyclooxygenase 2/PTGS2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3708)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase)

蛋白名全称

Prostaglandin G/H synthase 2

实验方案>

实验步骤

引用文献(0)>

清除