Anti-FGF2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

A00121-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U2OS whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: SK-OV-3 whole cell lysates,
Lane 4: Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (A00121-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF2 at approximately 17 kDa. The expected band size for FGF2 is at 31, 23, 17, 21 kDa.
all(5)
IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3).
FGF2 was detected in a paraffin-embedded section of human gastric cancer tissue. The tissue section was incubated with rabbit anti-FGF2 Antibody (A00121-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3).
FGF2 was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-FGF2 Antibody (A00121-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3).
FGF2 was detected in a paraffin-embedded section of rat cerebellum tissue. The tissue section was incubated with rabbit anti-FGF2 Antibody (A00121-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IF analysis of FGF2 using anti-FGF2 antibody (A00121-3).
FGF2 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-FGF2 Antibody (A00121-3) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(5)

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产品简介>

产品名称

Anti-FGF2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Basic fibroblast growth factor; BFGF; FGF2; FGF-2; FGF 2; FGFB; HBGF 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

FGF2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human FGF2, which shares 93.3% amino acid (aa) sequence identity with mouse and rat FGF2.

计算分子量

31, 23, 17, 21 kDa

实际分子量

17 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Uniprot ID

P09038

RRID

AB_3080961

文献引用格式

FGF2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00121-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

fibroblast growth factor 2

蛋白名全称

Fibroblast growth factor 2

实验方案>

实验步骤

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