Anti-FGF2 Antibody (Clone#OTI3D9)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF

MA00121

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF (Cat# MA00121).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00121)
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Anti-BFGF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF .
all(4)

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产品简介>

产品名称

Anti-FGF2 Antibody (Clone#OTI3D9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Basic fibroblast growth factor; BFGF; FGF2; FGF-2; FGF 2; FGFB; HBGF 2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

FGF2

克隆号

OTI3D9

宿主

Mouse

抗体亚型

IgG2b

免疫原

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

计算分子量

31, 23, 17, 21 kDa

实际分子量

31 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Uniprot ID

P09038

文献引用格式

FGF2 Antibody (Clone#OTI3D9) (Boster Biological Technology, Wuhan, China. Catalog#MA00121)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

fibroblast growth factor 2

蛋白名全称

Fibroblast growth factor 2

实验方案>

实验步骤

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