Anti-p21/CDKN1A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF ELISA

A00145-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P21/CDKN1A antibody (A00145-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P21/CDKN1A antigen affinity purified polyclonal antibody (A00145-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P21/CDKN1A at approximately 21 kDa. The expected band size for P21/CDKN1A is at 21 kDa.
all(5)
IHC analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1).
P21/CDKN1A was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1).
P21/CDKN1A was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1) and anti-Beta Tubulin antibody (M01857-3).
P21/CDKN1A was detected in an immunocytochemical section of Hela cells. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Dylight550-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1133) were used as secondary antibody.
all(5)
all(5)

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产品简介>

产品名称

Anti-p21/CDKN1A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CAP20; CDK interacting protein 1; CDKN1; CDKN1A; CIP1; MDA 6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, ELISA

基因名称

CDKN1A

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human P21/CDKN1A recombinant protein (Position: M1-R140).

计算分子量

18 kDa

实际分子量

21 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Uniprot ID

P38936

Gene ID

1026

文献引用格式

p21/CDKN1A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00145-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin dependent kinase inhibitor 1A

蛋白名全称

Cyclin-dependent kinase inhibitor 1

实验方案>

实验步骤

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