Anti-p21/CDKN1A Antibody (Clone#OTI5G1)

Mouse monoclonal antibody

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筛选器： All IHC WB

M00145-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00145-4) (1:150)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDKN1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00145-4) (1:150)
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-CDKN1A monoclonal antibody (1:200).
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Western blot analysis of extracts (35ug) from 8 cell lines by using anti-CDKN1A monoclonal antibody (1:250).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN1A (1:2000).
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产品简介>

产品名称

Anti-p21/CDKN1A Antibody (Clone#OTI5G1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CAP20; CDK interacting protein 1; CDKN1; CDKN1A; CIP1; MDA 6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

产品类型

Monoclonal

检验物种

human

应用范围

IHC, WB

基因名称

CDKN1A

克隆号

OTI5G1

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 2-164 of human CDKN1A(NP_510867) produced in E.coli.

计算分子量

18 kDa

实际分子量

17.9 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot ID

P38936

文献引用格式

p21/CDKN1A Antibody (Clone#OTI5G1) (Boster Biological Technology, Wuhan, China. Catalog#M00145-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cyclin-dependent kinase inhibitor 1

蛋白名全称

Cyclin-dependent kinase inhibitor 1

实验方案>

实验步骤

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