Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Ramos whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse EL4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD19 antigA03957-Aen affinity purified polyclonal antibody (A00154-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.
Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Ramos whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse EL4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD19 antigA03957-Aen affinity purified polyclonal antibody (A00154-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.