Anti-CD19 Antibody (Clone#OTI3G7)

Mouse monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF FCM

M00154-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

加入购物车立即购买

Western blot analysis of anti-CD19 antibody (M00154-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Ramos whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD19 antigen affinity purified monoclonal antibody (M00154-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 66 kDa.
all(4)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD19 mouse monoclonal antibody. (M00154-4; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
all(4)
Anti-CD19 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD19 .
all(4)
Flow cytometric Analysis of living K562 cells, using anti-CD19 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD19 Antibody (Clone#OTI3G7)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B lymphocyte antigen CD19; B4; CD19; CD19 molecule; Differentiation antigen CD19; T cell surface antigen Leu 12

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

CD19

克隆号

OTI3G7

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human CD19(NP_001761) produced in HEK293T cell.

计算分子量

66 kDa

实际分子量

95 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Uniprot ID

F1LNH2

文献引用格式

CD19 Antibody (Clone#OTI3G7) (Boster Biological Technology, Wuhan, China. Catalog#M00154-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD19 molecule

蛋白名全称

CD19 molecule

实验方案>

实验步骤

引用文献(0)>

清除