Anti-BAX DyLight 488 Conjugated Antibody

Rabbit polyclonal antibody

说明书

筛选器： All FCM

A00183-Dyl488

100μl ￥2380
货期： 5-7天

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Figure 1. Flow Cytometry analysis of A549 cells using anti-Human Bax antibody (A00183-Dyl488).Overlay histogram showing A549 cells stained with A00183-Dyl488 (Blue line).Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品名称

Anti-BAX DyLight 488 Conjugated Antibody

规格/价格

100μl/2380

指标别称

Apoptosis regulator BAX; BAX

产品类型

Polyclonal

检验物种

human

应用范围

FCM

基因名称

BAX

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Bax, different from the related mouse and rat sequences by five amino acids.

偶联物

DyLight 488

激发/发射光谱

Amax=488nm; Emax=515-545nm

成分

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

纯化方式

Immunogen affinity purified.

浓度

500ug/ml

产品形态

Liquid

保存条件

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

背景资料

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Uniprot ID

Q07812

RRID

AB_3080999

文献引用格式

BAX DyLight 488 Conjugated Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00183-Dyl488)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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实验步骤

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